LIFR Protein (C-His, Rat)

1.Measured by its binding ability in a functional ELISA. Immobilized Mouse LIF hFc (Cat: 50755-M02H) at 2 μg/ml (100 μl/well) can bind Rat LIFR His (Cat:80322-R08H), the EC50 of Rat LIFR His is 300-700 ng/mL. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant human LIF.

The recombinant rat LIFR comprises 797 amino acids and predicts a molecular mass of 90 kDa. The apparent molecular mass of the recombinant protein is approximately 116 kDa in SDS-PAGE under reducing conditions due to glycosylation.