LIF Protein (Native, Human)

Product Specifications

Abbreviation

LIF

Synonyms

CDF, DIA, HILDA, MLPLI

Host

HEK 293

Tag

Native

Endotoxin

< 10 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-0.8 ng/mL. 2.Immobilized Recombinant Human LIF Protein (Cat: 14890-HNAH) at 2 μg/mL (100 μL/well) can bind Recombinant Human LIFR Protein (Fc Tag) (Cat: 10628-H02H), the EC50 is 7-21 ng/mL. (Routinely tested)

Function

Met1-Phe202

Molecular Weight

The recombinant human LIF comprises 180 amino acids and has a predicted molecular mass of 19.7 kDa. The apparent molecular mass of the protein is approximately 23-43 kDa in SDS-PAGE under reducing conditions due to glycosylation.