LIF Protein (C-His, Human)

Product Specifications

Abbreviation

LIF

Synonyms

CDF, DIA, HILDA, MLPLI

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1.Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 2-15ng/mL. 2.Immobilized Recombinant Human LIF Protein (His Tag) (Cat: 14890-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human LIFR Protein (Fc Tag) (Cat: 10628-H02H), the EC50 is 8-24 ng/mL.

Function

Met1-Phe202

Molecular Weight

The recombinant human LIF comprises 191 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is approximately 35-42 kDa in SDS-PAGE under reducing conditions.