LGMN Protein (N-His, Mouse)

Product Specifications

Abbreviation

LGMN

Synonyms

AEP, Lgmn, Prsc1

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin (Z-AAN-AMC) . The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)

Function

Val18-Tyr435

Molecular Weight

The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.