KLK11 Protein (C-His, Human)
Product Specifications
Abbreviation
KLK11
Synonyms
IEKD, PRSS20, TLSP, UNQ649/PRO1279
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468) . The specific activity is >200 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Function
Met1-Asn250
Molecular Weight
The secreted recombinant human KLKL11 comprises 243 amino acids with a predicted molecular mass of 27 kDa. As a result of glycosylation, rhKLK11 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
Species
Human
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