KLK1 Protein (C-His, Human)

Product Specifications

Abbreviation

KLK1

Synonyms

HK1, Klk6, KLKR

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) . The specific activity is >1,500 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Function

Met1-Ser262

Molecular Weight

The secreted recombinant human KLK1 is a monomeric protein after cleavage of the signal peptide. It consists of 251 amino acids and predictes a molecular mass of 28 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.