KLB Protein (C-His, Mouse)

Product Specifications

Abbreviation

KLB

Synonyms

Betakl, betaKlotho, Klb

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human FGF21 Protein (Cat: 10911-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse Klotho beta Protein (His Tag) (Cat: 50661-M08H1), the EC50 is 100-300 ng/mL. 2.Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2-2 μg/mL in the presence of Recombinant human FGF21.

Function

Phe53-Leu995

Molecular Weight

The recombinant Mouse KLB consisting of 954 amino acids and has a calculated molecular mass of 110.39 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 59 kDa protein in SDS-PAGE under reducing conditions.