KIT Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

KIT

Synonyms

Bs, c-KIT, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, SCO1, SCO5, Sl, SOW3, Ssm, Tr-kit, W

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1.Immobilized Recombinant Mouse SCF / C-kit ligand Protein (ECD, His Tag) (Cat: 50487-M08B) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse c-kit / CD117 Protein (Fc Tag) (Cat: 50530-M02H), the EC50 is 8-24 ng/mL (QC tested) . 2.Immobilized Recombinant Human SCF / C-kit ligand Protein (aa 1-189, His Tag) (Cat: 10451-H08B) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse c-kit / CD117 Protein (Fc Tag) (Cat: 50530-M02H), the EC50 is 0.6-1.8 μg/mL (Routinely tested) .

Function

Met1-Thr523

Molecular Weight

The secreted recombinant mouse KIT/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 740 amino acids and has a predicted molecular mass of 82.5 kDa. As a result of glycosylation, the apparent molecular mass of rmKIT/Fc monomer is approximately 100-110 kDa in SDS-PAGE under reducing conditions.