JAM2 Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

JAM2

Synonyms

1110002N23Rik,2410030G21Rik,2410167M24Rik, JAM-2, JAM-B, Jam2, Jcam2, VE-JAM, Vejam

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 70 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells . When 8 x 10E4 cells/well are added to JAM2-coated plates (0.2 μg/ml and 100 μl/well), approximately 40-55% will adhere specifically after 60 minutes at 37°C.

Function

Met1-Asn236

Molecular Weight

The secreted recombinant mouse JAM-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 449 amino acids and has a predicted molecular mass of 50.3 kDa. rm JAM-2/Fc monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.