JAG1 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

JAG1

Synonyms

AGS, AGS1, AHD, AWS, CD339, CMT2HH, DCHE, HJ1, JAGL1

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 85 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-30 μg/mL.

Function

Met1-Ser1046

Molecular Weight

The recombinant human JAG1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 1254 amino acids and has a predicted molecular mass of 137 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human JAG1/Fc monomer is approximately 185.5 kDa due to glycosylation.