INSR Protein (C-His-AVI, Human)

Product Specifications

Abbreviation

INSR

Synonyms

CD220, HHF5

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Biotinylated Recombinant Human Insulin Receptor Protein (short isoform), His & AVI Tag (Cat. No. 11086-H49H-B) captured on SA chip can bind Insulin with an affinity constant of 0.296 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 2.Human Insulin Receptor Protein (short isoform), His & AVI Tag (Cat. No. 11086-H49H-B) captured on CM5 chip via Anti-His antibody, can bind Insulin with an affinity constant of 0.438 μM as determined in an SPR assay (Biacore T200) (QC tested) .

Function

Met1-Lys944

Molecular Weight

The recombinant Human INSR consists of 943 amino acids and predicts a molecular mass of 107.9 kDa. It migrates as an approximately 168.3 kDa, 120kDa and 54.8kDa band in SDS-PAGE under reducing conditions.