INHBA Protein (C-His, Mouse)

1. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His (Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:50659-M08H) is 0.161 μg/mL. 2. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL. 3. Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is typically 3-30 μg/mL. 4.Recombinant Human ACVR2B Protein, His Tag (Cat. No. 10229-H08H) immobilized on CM5 chip can bind Recombinant Mouse Activin A Protein, His Tag (Cat. No. 50659-M08H) with an affinity constant of 2.420 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Recombinant Mouse ACVR2B Protein, His Tag (Cat. No. 50173-M08H) immobilized on CM5 chip can bind Recombinant Mouse Activin A Protein, His Tag (Cat. No. 50659-M08H) with an affinity constant of 3.470 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The secreted recombinant mouse INHBA comprises 415 amino acids and has a calculated molecular mass of 46.5 kDa. As a result of glycosylation, the apparent molecular mass of rmINHBA is approximately 18 & 43 kDa corresponding to the mature inhibin beta A chain and the pro domain respectively in SDS-PAGE under reducing conditions.