IL7 Protein (C-His, Cynomolgus)

Product Specifications

Abbreviation

IL7

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC) . The ED50 for this effect is typically 5.1ng/ml (Routinely tested) .

Function

Met1-His178

Molecular Weight

The recombinant Cynomolgus IL7 consists of 164 amino acids and predicts a molecular mass of 19 kDa. It migrates as an approximately 25-30 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 27.0 kDa verified by SEC-MALS (Routinely tested) .