IL6ST Protein (C-mouse IgG1-Fc & His, Rat)

Product Specifications

Abbreviation

IL6ST

Synonyms

Ac1055, Gp130, Il-6rb, Il6st

Host

HEK 293

Tag

C-mouse IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.) . The ED50 for this effect is typically 0.015-0.075 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.

Function

Met4-Glu618

Molecular Weight

The recombinant rat IL6ST/Fc is a disulfide-linked homodimer. The reduced monomer comprises 839 amino acids and predicts a molecular mass of 94.3 kDa. The apparent molecular mass of the rat IL6ST/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions due to glycosylation.