IL6ST Protein (C-human IgG1-Fc & His, Human)

Product Specifications

Abbreviation

IL6ST

Synonyms

CD130, CDW130, GP130, HIES4, HIES4A, HIES4B, IL-6RB, IMD94, sGP130, STWS2

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cell. The ED50 for this effect is typically 0.2-0.8 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.

Function

Met1-Ile618

Molecular Weight

The recombinant human IL6ST/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 844 amino acids and has a predicted molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL6ST/Fc monomer is approximately 125-140 kDa due to glycosylation.