IL6R Protein (C-human IgG1-Fc, Human)

1.Measured by its ability to enhance the activity of IL6 on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 30-125 ng/mL in the presence of 100 ng/mL of recombinant human IL6. 2.Loaded Recombinant Human IL-6R Protein, hFc Tag (Cat. No. 10398-H02H) on ProA Biosensor, can bind Recombinant Human IL-6 Protein, no Tag (Cat. No. 10395-HNAE) with an affinity constant of 0.51μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human IL6 / Interleukin-6 Protein (Cat: 10395-HNAE) at 2μg/mL (100μL/well) can bind Recombinant Human IL-6R / CD126 Protein (ECD, Fc Tag) (Cat: 10398-H02H), the EC50 is 5-15 ng/mL. 4.Recombinant Human IL-6R / CD126 Protein (ECD, Fc Tag) (Cat: 10398-H02H) captured on Protein A chip, can bind Recombinant Human IL-6 protein (Cat: 10395-HNAH) with an affinity constant of 65.470 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human IL6R consists of 584 amino acids and predicts a molecular mass of 65.3 kDa. As a result of glycosylation, it migrates as an approximately 70-100 kDa band in SDS-PAGE under reducing conditions.