IL6R Protein (C-His-AVI, Human)

Product Specifications

Abbreviation

IL6R

Synonyms

CD126, gp80, HIES5, IL-1Ra, IL-6R, IL-6R-1, IL-6RA, IL6Q, IL6QTL, IL6RA, IL6RQ

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R, Biotinylated (Cat:10398-H49H-B), the EC50 of Human IL-6R, Biotinylated (Cat:10398-H49H-B) is 20-160 ng/mL. 2.Immobilized Recombinant Human IL-6 protein (Cat: 10395-HNAH) at 10 μg/mL (100 μl/well) on Recombinant Human IL6ST / gp130 / CD130 Protein (ECD, Fc Tag) (Cat: 10974-H02H) precoated (5 μg/mL, 100 μL/well) can bind Recombinant Human IL-6R Protein (His & AVI Tag), Biotinylated (Cat: 10398-H49H-B), the EC50 is 4-12 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human IL-6 protein (Cat: 10395-HNAH) at 10 μg/mL (100 μl/well) on Recombinant Rhesus IL6ST / gp130 Protein (Fc Tag) (Cat: 90275-C02H) precoated (5 μg/mL, 100 μL/well) can bind Recombinant Human IL-6R Protein (His & AVI Tag), Biotinylated (Cat: 10398-H49H-B), the EC50 is 5-15 ng/mL (Routinely tested) .

Function

Met1-Pro365

Molecular Weight

The recombinant human IL6R consists of 372 amino acids and predicts a molecular mass of 41.80 kDa. It migrates as an approximately 60.56 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 72.0 kDa verified by SEC-MALS (Routinely tested) .

Species

Human