IL6R & IL6 Protein (N-His, Human)

Product Specifications

Abbreviation

IL6R & IL6

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human IL-6/IL-6R alpha Chimera Protein (His Tag) (Cat: CC001-H07H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL6ST / gp130 / CD130 Protein (ECD, Fc Tag) (Cat: 10974-H02H), the EC50 is 1-5 ng/mL (QC tested) . 2.Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 1-6ng/mL.

Function

Leu20-Asp358 & Val30-Met212

Molecular Weight

The recombinant Human IL-6/IL-6R alpha Chimera consists of 556 amino acids and predicts a molecular mass of 61.95 kDa. It migrates as an approximately 82.5 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 81.9 kDa verified by SEC-MALS (Routinely tested) .