IL5 Protein (C-His-AVI, Human)
Product Specifications
Abbreviation
IL5
Synonyms
EDF, IL-5, TRF
Host
HEK 293
Tag
C-His-AVI
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
Bioactivity
1.Immobilized Recombinant Human IL-5 Protein (His & AVI Tag), Biotinylated (Cat: 15673-H49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL-5R alpha Protein (Membrane-bound, Fc Tag) (Cat: 10392-H01H1), the EC50 is 20-60 ng/mL. 2.Loaded Biotinylated Recombinant Human IL-5 Protein, His & AVI Tag (Cat. No. 15673-H49H-B) on SA Biosensor, can bind Recombinant Human IL-5R alpha Protein, Membrane-bound, Fc Tag (Cat. No. 10392-H01H1) with an affinity constant of 2.320 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Biotinylated Recombinant Human IL-5 Protein, His & AVI Tag (Cat. No. 15673-H49H-B) on SA Biosensor, can bind Recombinant Human IL-5R alpha Protein, Membrane-bound, His Tag (Cat. No. 10392-H08H1) with an affinity constant of 17.800 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ile20-Ser134
Molecular Weight
The recombinant Human IL5 comprises 141 amino acids and has a predicted molecular mass of 16.41 kDa. It migrates as approximately 21 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 39.3 kDa verified by SEC-MALS.
Species
Human
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