IL4 Protein (C-His, Mouse)

1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His (Cat:51084-M08B) at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc (Cat:80198-R02H), The EC50 of rat IL4R-Fc (Cat:80198-R02H) is 0.12-0.29 μg/mL. 2.Immobilized Recombinant Mouse IL4 / Interleukin-4 Protein (Q136D, Y139D, His Tag) (Cat: 51084-M08B) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse IL4R Protein (Fc Tag) (Cat: 51180-M02H), the EC50 is 1.0-3.5 ng/mL (QC tested) . 3. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.

The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation.