IL33 Protein (N-His & AVI, Human)

Product Specifications

Abbreviation

IL33

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV, NFEHEV, NFHEV

Host

E. coli

Tag

N-His & AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human IL1RL1 / DER4 Protein (Fc Tag) (Cat: 10105-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-33 Protein (His & AVI Tag), Biotinylated (Cat: 10368-H56E-B), the EC50 is 1.0-4.0 ng/mL (QC tested) . 2.Loaded Recombinant Human ST2/IL-1 RL1 Protein, hFc Tag (Cat. No. 10105-H02H) on ProA Biosensor, can bind Recombinant Human IL-33 Protein, His & AVI Tag (Cat. No. 10368-H56E-B) with an affinity constant of 1.730 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Ser112-Thr270

Molecular Weight

The recombinant Human IL33 comprises 205 amino acids and has a predicted molecular mass of 22.31 kDa. It migrates as approximately 26.7 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 50.8 kDa verified by SEC-MALS (Routinely tested) .