IL33 Protein (Native, Human)

Product Specifications

Abbreviation

IL33

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV, NFEHEV, NFHEV

Host

E. coli

Tag

Native

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Immobilized Recombinant Human IL1RL1 / DER4 Protein (Fc Tag) (Cat: 10105-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL33 / Interleukin-33 / NF-HEV Protein (Cat: 10368-HNAE), the EC50 is 60-180 ng/mL (QC tested) . 2. Measured by its ability to induce IL8 secretion by human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 15-150ng/mL. 3.Loaded Recombinant Human IL1RL1 / DER4 Protein (Fc Tag) (Cat. No. 10105-H02H) on ProA Biosensor, can bind Recombinant Human IL33 / Interleukin-33 / NF-HEV Protein (Cat. No. 10368-HNAE) with an affinity constant of 3.47 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Ser112-Thr270

Molecular Weight

The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.

Species

Human