IL31 Protein (C-His, Human)

1.Measured by its ability to induce STAT3 activation in U‐87 cells. The ED50 for this effect is 11-45 ng/mL. 2.Immobilized Recombinant Human IL-31 / IL31 Protein (His Tag) (Cat: 11557-H08H) at 1 μg/mL (100 μL/well) can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD), the EC50 is 0.85 μg/mL (Routinely tested) . 3.Recombinant Human IL-31 / IL31 Protein (His Tag) (Cat: 11557-H08H) immobilized on CM5 chip, can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (His Tag) (Cat: 15878-H08H) with an affinity constant of 34.100 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human IL31 consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 31.8 KDa band in SDS-PAGE under reducing conditions due to glycosylation.