IL2RB Protein (C-human IgG1-Fc, Human)

1. Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells (Routinely tested) . 2.Recombinant Human IL-2R beta/CD122 Protein, hFc Tag (Cat. No. 10696-H02H) captured on Protein A chip can bind Recombinant Human IL-15 Protein (Cat. No. 10360-HNCE) with an affinity constant of 4.48 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Loaded Recombinant Human IL-2R beta/CD122 Protein, hFc Tag (Cat. No. 10696-H02H) on proA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 268 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human CD122 / IL-2RB Protein (ECD, Fc Tag) (Cat: 10696-H02H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E) with an affinity constant of 0.551 μM as determined in an SPR assay (Biacore 8K) (QC tested) . 5.Immobilized Recombinant Human IL-15 / IL15 / Interleukin 15 Protein (Cat: 10360-HNCE) at 5 μg/mL (100 μL/well) can bind Recombinant Human CD122 / IL-2RB Protein (ECD, Fc Tag) (Cat: 10696-H02H), the EC50 is 79.3 ng/mL (Routinely tested) .

The recombinant human IL2 Rβ/Fc is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51.3 kDa. As a result of glycosylation, the apparent molecular mass of rh IL2RB/Fc monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions.