IL2RB & IL2RG Protein (C-Flag & C-His-AVI, Human)
Product Specifications
Abbreviation
IL2RB & IL2RG
Host
HEK 293
Tag
C-Flag & C-His-AVI
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 2.190 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAH1-E) with an affinity constant of 1.210 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .
Function
Ala27-Asp239 & Leu23-Asn254
Molecular Weight
The recombinant heterodimer of human IL2RB & IL2RG comprises 616 (313+303) amino acids and has a calculated molecular mass of 68.34 (34.25+34.09) KDa. The apparent molecular mass of human IL2RB & IL2RG heterodimer is approximately 59 kDa and 45 kDa respectively in SDS-PAGE. The molecular weight of this protein is around 98.7 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
Curated Selection
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