IL2RA Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

IL2RA

Synonyms

CD25, IDDM10, IL2R, IMD41, p55, TCGFR

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human IL2 Protein, Low Endotoxin (Cat:11848-HNAH1-E) at 2 μg/ml (100 μl/well) can bind CD25/IL2RA Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10165-H02H), The EC50 is 3-10 ng/mL (QC tested) . 2.Recombinant Human IL2Ra / CD25 Protein (ECD, Fc Tag) (Cat: 10165-H02H) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E) with an affinity constant of 17.600 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Cys213

Molecular Weight

The mature recombinant human IL2Rα/Fc is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The reduced monomer consists of 430 amino acids and has a predicted molecular mass of 48.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα/Fc monomer is approximately 72 kDa due to glycosylation.