IL27 Protein (C-His, Mouse)

Product Specifications

Abbreviation

IL27

Synonyms

IL-27, IL-27-A, IL-27p28, Il27, IL27-A, Il27a, Il30, p28

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.26-0.62 μg/ml. 2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is 1-10 ng/ml.

Function

Met1-Pro228 & Phe29-Ser234

Molecular Weight

The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.