IL27 Protein (C-His, Human)

Product Specifications

Abbreviation

IL27

Synonyms

IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28

Host

CHO

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.2-0.46 μg/ml. 2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is 10-60 ng/mL.

Function

Met1-Lys229 & Phe29-Pro243

Molecular Weight

The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.