IL25 Protein (N-His, Mouse)

Product Specifications

Abbreviation

IL25

Synonyms

IL-17e, IL-25, Il17e, Il25

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 80 % as determined by SDS-PAGE

Bioactivity

1. Immobilized IL17RB Protein, Mouse, Recombinant (hFc Tag) (Cat:51109-M02H) at 2 μg/mL (100 μL/well) can bind IL25/IL17E Protein, Mouse, Recombinant (His Tag) (Cat:50138-M07H), the EC50 is 4-20 ng/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 4-14ng/mL.

Function

Val 17-Ala 169

Molecular Weight

The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.