IL25 Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

IL25

Synonyms

IL-17E, IL-25, IL17E, UNQ3120/PRO10272

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His (Cat:13091-H08H) at 10 μg/ml (100 μl/well) can bind human Fc-IL25 (Cat:10096-H01H), The EC50 of human Fc-IL25 (Cat:10096-H01H) is 1.7-4.2 μg/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL.

Function

Tyr 33-Gly177

Molecular Weight

The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 425 amino acids and predicts a molecular mass of 47.55 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions.