IL1R2 Protein (C-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/ml (100ul/well) can bind recombinant human IL1R2-Fc with a linear range of 6.25-400 ng/ml (QC tested) . 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. 10111-H02H) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM (Routinely tested) . 3. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The EC50 for this effect is typically 0.1-0.8 μg/mL (Routinely tested) . 4.Loaded Recombinant Human IL1R2 / CD121b Protein (Fc Tag) (Cat. No. 10111-H02H) on ProA Biosensor, can bind Recombinant Human IL-1 beta / IL1B Protein (Cat. No. 10139-HNAE) with an affinity constant of 6.0 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Recombinant Human IL1R2 / CD121b Protein (Fc Tag) (Cat: 10111-H02H) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human IL-1 beta / IL1B Protein (Cat: 10139-HNAE) with an affinity constant of 4.720 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 568 amino acids and has a predicted molecular mass of 64.5 kDa. As a result of glycosylation, the rh IL1R2/Fc monomer migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.