IL1R2 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

IL1R2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Canine IL1R2-Fc3 at 10 μg/ml (100 μl/well) can bind biotinylated human IL1B-His (Cat:10139-H07E), The EC50 of biotinylated human IL1B-His (Cat:10139-H07E) is 0.08-0.2 μg/ml.

Function

Met1-Glu343

Molecular Weight

The recombinant canine IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 571 amino acids and has a predicted molecular mass of 64.8 kDa. The apparent molecular mass of the protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.