IL1R1 Protein (C-His, Mouse)

1. Measured by its ability to bind mouse IL1B in a functional ELISA. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL. 2. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 20 ng/mL rmIL1α. The EC50 for this effect is typically 20-80 ng/mL.

The secreted recombinant mouse IL1R1 comprises 330 amino acids and has a calculated molecular mass of 38.7 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.