IL18RAP Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

IL18RAP

Synonyms

ACPL, CD218b, CDw218b, IL-18R-beta, IL-18RAcP, IL-18Rbeta, IL-1R-7, IL-1R7, IL-1RAcPL, IL18RB, IL1R7

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Recombinant Human IL-18R beta / IL-18RAP Protein, hFc Tag (Cat. No. 10176-H02H) captured on Protein A chip can bind Recombinant Human IL-18 Protein (Cat. No. 10119-HNCE) (Premixed with Recombinant Human IL-18Rα Protein, His Tag, Cat. No. 11102-H08H) with an affinity constant of 12.85 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Gly357

Molecular Weight

The recombinant human IL18RAP/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 575 amino acids and has a calculated molecular mass of 65.2 kDa. As a result of glycosylation, the apparent molecular mass of rhIL18RAP/Fc monomer is approximately 80-90 kDa in reduced SDS-PAGE.