IL18R1 Protein (C-mouse IgG2a-Fc, Mouse)

1.Measured by its binding ability in a functional ELISA. Immobilized mIL18R1-mFc (mIgG2a) (Cat:50094-M38H) at 10 μg/mL (100 μL/well) can bind mIL18/Biotin (Cat:50073-MNCE), the EC50 of mIL18/Biotin (Cat:50073-MNCE) is 90-260 ng/mL. 2.Loaded Recombinant Mouse IL-18R beta / IL-18RAP Protein, His Tag (Cat. No. 50845-M08B) on His1K Biosensor, can bind Recombinant Mouse IL-18Rα Protein, mFc Tag (Cat. No. 50094-M38H) (Premixed with Recombinant Mouse IL-18 Protein, Tag Free, Cat. No. 50073-MNCE) with an affinity constant of 8.65 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .