IL17A Protein (Native, Mouse)

1. Measured by its binding ability in a functional ELISA. Immobilized mouse Il17A (Cat: 51065-MNAE) at 2 μg/mL (100 μl/well) can bind mouse IL17RA-His (Cat: 50328-M08H), The EC50 of mouse IL17RA-His (Cat:50328-M08H) is 12-45 ng/mL. 2. Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50 for this effect is 0.15-0.6 ng/mL. 3.Loaded Recombinant Human IL-17RA Protein, hFc Tag (Cat. No. 10895-H02H) on ProA Biosensor, can bind Recombinant Mouse IL-17A Protein (Cat. No. 51065-MNAE) with an affinity constant of 18.880 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mouse IL17a consists of 138 amino acids and predicts a molecular mass of 15.5 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.