IL17A Protein (Native, Marmoset)

1. Measured by its binding ability in a functional ELISA. Immobilized Marmoset IL17A (cat: 90287-CNAE) at 10 μg/mL (100 μl/well) can bind human IL17Ra (Cat:10895-H03H), The EC50 of human IL17Ra (Cat:10895-H03H) is 8.5-20.0 ng/mL. 2. Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50 for this effect is 0.5-2.5 ng/mL.

The recombinant Marmoset IL17A consists of 135 amino acids and has a calculated molecular mass of 15.4 kDa. It migrates as an 17 kDa band in SDS-PAGE under reducing conditions.