IL17A Protein (N-His & AVI, Human)

Product Specifications

Abbreviation

IL17A

Synonyms

CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17

Host

HEK 293

Tag

N-His & AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human IL-17R / IL-17RA (CD217) Protein (Fc Tag) (Cat: 10895-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL17 Protein (His & AVI Tag), Biotinylated (Cat: 12047-H44H-B), the EC50 is 4-12 ng/mL. 2.Anti-IL17A (Research Grade Ixekiz*mab Biosimilar) captured on Protein A chip, can bind Biotinylated Recombinant Human IL-17A Protein, AVI & His Tag (Cat. No. 12047-H44H-B) with an affinity constant of 0.112 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Anti-IL17A (Research Grade Secukin*mab Biosimilar) captured on Protein A chip, can bind Biotinylated Recombinant Human IL-17A Protein, AVI & His Tag (Cat. No. 12047-H44H-B) with an affinity constant of 0.848 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Gly24-Ala155

Molecular Weight

The recombinant Human IL17A consists of 157 amino acids and predicts a molecular mass of 18.31 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 41.6 kDa、24.6 kDa、22.5 kDa、21.1 kDa、19.4 kDa and 17.4 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 43.6 kDa verified by SEC-MALS (Routinely tested) .