IL13Ra2 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

IL13Ra2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.01-0.04 μg/mL. 2.Immobilized Recombinant Canine IL13RA2 / IL13R Protein (Fc Tag) (Cat: 70079-D02H) at 1 μg/mL (100 μL/well) can bind Anti- IL-13 R alpha 2/IL13RA2 antibody, human IgG1, the EC50 is 4.6-13.8 ng/mL (QC tested) .

Function

Met1-Thr338

Molecular Weight

The recombinant canine IL13RA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 558 amino acids and has a predicted molecular mass of 64.1 kDa. The apparent molecular mass of the protein is approximately 66-72 kDa in SDS-PAGE under reducing conditions.