IL13Ra2 Protein (C-human IgG1-Fc & His, Mouse)

Product Specifications

Abbreviation

IL13Ra2

Synonyms

CD213a2, IL-13R-alpha-2, Il13ra2

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Immobilized human IL13 at 20 μg/ml (100 μl/well) can bind mouse IL13RA2 with a linear range of 0.5-2 μg/ml. 2. Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/ml.

Function

Met1-Lys334

Molecular Weight

The recombinant mouse IL13Rα2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 561 amino acids and has a predicted molecular mass of 64.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIL13Rα2/Fc monomer is approximately 75-80 kDa due to glycosylation.