IL13 Protein (N-His, Cynomolgus)

Product Specifications

Abbreviation

IL13

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized cynomolgus IL-13 at 4 μg/ml (100 μl/well) can bind human IL13RA1 with a linear range of 0.156-2.5 μg/ml. 2. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 100-400 ng/mL.

Function

Ser21-Asn132

Molecular Weight

The recombinant cynomolgus IL13 consists of 128 amino acids and has a calculated molecular mass of 14.5 kDa. The apparent molecular mass of the protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.