IL13 Protein (Native, Human)

1.Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.) . The ED50 for this effect is typically 0.5-3 ng/mL. 2.Loaded Recombinant Human IL-4Rα & IL-13Rα1 Heterodimer Protein, hFc Tag (Cat. No. CT117-H02H) on proA Biosensor, can bind Recombinant Human IL-13 Protein (Cat. No. 10369-HNAC) with an affinity constant of 1.690 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human IL13RA1 Protein (ECD, Fc Tag) (Cat. No. 10943-H02H) on ProA Biosensor, can bind Recombinant Human IL13 Protein (Cat. No. 10369-HNAC) with an affinity constant of 17.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human IL13RA1 Protein (ECD, Fc Tag) (Cat: 10943-H02H) captured on Protein A chip, can bind Recombinant Human IL13 Protein (Cat: 10369-HNAC) with an affinity constant of 9.560 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Recombinant Human IL13RA1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10943-H49H-B) captured on NTA chip, can bind Recombinant Human IL13 Protein (Cat: 10369-HNAC) with an affinity constant of 3.366 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human IL13 consists of 112 amino acids and migrates as an approximately 12.35 kDa band in SDS-PAGE under reducing conditions as predicted.