IL12B Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

IL12B

Synonyms

Il-12b, Il-12p40, Il12b, Il12p40, p40

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse IL12B-Fc at 10μg/mL (100μL/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H) . The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 0.10-0.24μg/mL.

Function

Met1-Ser335

Molecular Weight

The secreted recombinant mouse IL12B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 554 amino acids and has a calculated molecular mass of 62.8 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 83 kDa in SDS-PAGE under reducing conditions.