IL12B Protein (C-His, Rat)

Product Specifications

Abbreviation

IL12B

Synonyms

Il12

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized rat IL12B (cat: 80352-R08H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse IL12RB2 (Cat:50099-M08H) . The EC50 of biotinylated mouse IL12RB2 (Cat:50099-M08H) is 0.6-1.5 μg/ml.

Function

Met1-Ser335

Molecular Weight

The recombinant rat IL12B comprises 324 amino acids and predicts a molecular mass of 37.2 kDa. The apparent molecular mass of the recombinant protein is approximately 47 and 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.