IL12B & IL23A Protein (C-His, Human, Mouse)

1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL can bind Recombinant Human IL23A & Mouse IL12B Heterodimer Protein (His Tag) (Cat: CT155-A08H), the EC50 is 5-15 ng/mL (QC tested) . 2.Immobilized Recombinant Human IL23A & Mouse IL12B Heterodimer Protein (His Tag) (Cat: CT155-A08H) at 2 μg/mL can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 80-250 ng/mL (Routinely tested) . 3.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Human IL23A & Mouse IL12B Heterodimer Protein, His Tag (Cat. No. CT155-A08H) with an affinity constant of 5.100 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Human IL-12 R beta 1/IL12RB1 Protein, hFc Tag (Cat. No. 11674-H02H) on proA Biosensor, can bind Human IL23A & Mouse IL12B Heterodimer Protein, His Tag (Cat. No. CT155-A08H) with an affinity constant of 38.800 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant heterodimer of Human IL23A & Mouse IL12B comprises 494 (313+181) amino acids and has a calculated molecular mass of 55.93 (35.8+20.13) kDa. As a result of glycosylation, the apparent molecular mass of Human IL23A & Mouse IL12B Heterodimer is approximately 45.5 kDa、42.2 kDa and 21.7 kDa in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 61.4 kDa verified by SEC-MALS.