IL12B & IL23A Protein (C-His, Human)

1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 Heterodimer Protein (His Tag) (Cat: CT139-H08H), the EC50 is 3-12 ng/mL (QC tested) . 2.Immobilized Recombinant Human IL-23 Heterodimer Protein (His Tag) (Cat: CT139-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 70-260 ng/mL (Routinely tested) . 3.Immobilized Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 Heterodimer Protein (His Tag) (Cat: CT139-H08H), the EC50 is 3-10 ng/mL (Routinely tested) . 4.Immobilized Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 Heterodimer Protein (His Tag) (Cat: CT139-H08H), the EC50 is 3-10 ng/mL (Routinely tested) . 5.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT139-H08H) with an affinity constant of 4.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.6.Loaded Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) on ProA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT139-H08H) with an affinity constant of 0.579 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Loaded Recombinant Human IL-12 R beta 1/IL12RB1 Protein, hFc Tag (Cat. No. 11674-H02H) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT139-H08H) with an affinity constant of 16.1 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Loaded Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT139-H08H) with an affinity constant of 0.231 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 9.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is <4 ng/mL (QC tested) .

The recombinant heterodimer of human IL12B & IL23A comprises 487 (306+181) amino acids and has a calculated molecular mass of 54.83 (34.7+20.13) KDa. The apparent molecular mass of human IL12B & IL23A heterodimer is approximately 43.6 kDa、39.8 kDa and 21.1 kDa respectively in SDS-PAGE. The molecular weight of this protein is around 57.0 kDa verified by SEC-MALS (Routinely tested) .