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IL12B & IL23A Protein (C-AVI & His, Human)

Product Specifications

Abbreviation

IL12B & IL23A

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H), the EC50 is 8-30 ng/mL. 2.Immobilized Recombinant Human IL23 Protein (His & AVI Tag), Biotinylated (Cat: CT048-H27H-B) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 1-3 ng/mL (Routinely tested) . 3.Loaded Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) on SA Biosensor, can bind Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) with an affinity constant of 65.7 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) on SA Biosensor, can bind Recombinant Human IL-12 R beta 1/IL12RB1 Protein, hFc Tag (Cat. No. 11674-H02H) with an affinity constant of 60.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Loaded Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.551nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) on proA Biosensor, can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 1.25nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Anti-IL-23 Antibody (P19) (Research Grade Risankiz*mab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.319 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 8.Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) captured on Protein A chip can bind Biotinylated Recombinant Human IL-23 Protein, His & AVI Tag (Cat. No. CT048-H27H-B) with an affinity constant of 0.669 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Ser328 & Arg20-Pro189

Molecular Weight

The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.

Species

Human

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