IL12B & IL23A Protein (C-His, Human)

1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) (Cat: CT012-H08H), the EC50 is 120-400 ng/mL (QC tested) . 2.Immobilized Recombinant Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) (Cat: CT012-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 200-600 ng/mL (Routinely tested) . 3.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 1-10 ng/mL (QC tested) . 4.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT012-H08H) with an affinity constant of 18.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Loaded Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT012-H08H) with an affinity constant of 0.532 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181+317) amino acids and has a calculated molecular mass of 56.3 (20.1+36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22.6 & 42.8 kDa respectively in SDS-PAGE under reducing conditions.