IL12B & IL23A Protein (C-His, Cynomolgus, Rhesus)
Product Specifications
Abbreviation
IL12B & IL23A
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.≥ 90% as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Immobilized Recombinant Cynomolgus / Rhesus IL23R / IL23 Receptor Protein (Fc Tag) (Cat: 90123-C02H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus IL-23 Heterodimer Protein (His Tag) (Cat: CT146-C08H), the EC50 is 8-24 ng/mL. 2.Immobilized Recombinant Cynomolgus IL-23 Heterodimer Protein (His Tag) (Cat: CT146-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 20-60 ng/mL (Routinely tested) . 3.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Cynomolgus IL-23 Protein, His Tag (Cat. No. CT146-C08H) with an affinity constant of 5.75nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ile23-Ser328 & Val22-Pro189
Molecular Weight
The recombinant heterodimer of Cynomolgus / Rhesus IL-23 comprises 492 (306+186) amino acids and has a calculated molecular mass of 55.09 (34.77+20.32) kDa. As a result of glycosylation, the apparent molecular mass of Cynomolgus IL-23 Heterodimer is approximately 43.9 kDa、40.5 kDa and 22.7 kDa in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 60.9 kDa verified by SEC-MALS.
Species
Cynomolgus, Rhesus
Curated Selection
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