IL12A & IL12B Protein (C-His, Human)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

1.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL. 2.Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H) captured on Protein A chip, can bind Recombinant Human IL-12 (IL12A&IL12B Heterodimer) Protein (His Tag) (Cat: CT050-H08H) with an affinity constant of 4.108 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Immobilized Recombinant Human IL-12 (IL12A&IL12B Heterodimer) Protein (His Tag) (Cat: CT050-H08H) at 0.5 μg/mL (100 μL/well) can bind Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar), the EC50 is 1.0-4.0 ng/mL (Routinely tested) .